Showing : Single Cell • reset
15
votes
12
replies
2.5k
views
ZINB-WaVE + DESeq2 misses low-expressed, single-condition genes that look like they should be markers
deseq2 ZINB-WaVE single cell RNA-seq differential expression
updated 5.8 years ago by 42k • written 5.8 years ago by ▴ 60
13
votes
21
replies
6.1k
views
modeling zero-dominated RNA-seq with voom/limma and hurdle models (pscl)
limma voom hurdle single cell
8.7 years ago osa.zuta ▴ 40
8
votes
8
replies
4.1k
views
Disagreement between TMM (edgeR) and RLE (DESeq) normalisation for single-cell data
rnaseq single cell normalization edgeR deseq2
updated 7.7 years ago by ★ 28k • written 7.7 years ago by ▴ 50
7
votes
7
replies
5.3k
views
What are the methods to get count data per cell from single cell fastq given only R1, R2, and I1 fastq files
fastq single cell
updated 5.2 years ago by 50k • written 5.2 years ago by ▴ 360
6
votes
2
replies
5.3k
views
Cell type specific cell markers for Seurat based analysis.
single cell seurat clustering
updated 6.8 years ago by ▴ 40 • written 6.8 years ago by ▴ 20
4
votes
5
replies
1.8k
views
SC3 gene clusters
SC3 single cell single-cell Tutorial
updated 6.6 years ago by 25k • written 6.6 years ago by ▴ 20
4
votes
0
replies
3.4k
views
Tutorial: Workflow for low-level analysis of single-cell RNA-seq data
scran scater single cell Tutorial
7.7 years ago Aaron Lun ★ 28k
3
votes
3
replies
1.3k
views
MNN batch correction of pre-computed PCA
single cell batchelor
updated 4.1 years ago by ★ 28k • written 4.1 years ago by ▴ 10
3
votes
6
replies
3.9k
views
R package for single-cell RNA-seq data from fastq to matrix
single cell
updated 4.2 years ago by ★ 28k • written 4.2 years ago by ▴ 20
3
votes
6
replies
3.2k
views
Pool Single-cells: Analysis
deseq2 single cell limma voom
updated 8.1 years ago by ★ 28k • written 8.1 years ago by ▴ 90
3
votes
3
replies
1.5k
views
Different number of marker genes between same clusters
sce markers single cell DE clusters
4.6 years ago ludmi.b • 0
3
votes
1
reply
1.4k
views
SC3 with latent variable corrected counts
SC3 single cell
updated 7.6 years ago by ▴ 150 • written 7.6 years ago by ▴ 10
3
votes
4
replies
3.2k
views
Single cells batch effects
RUV single cell batch effect
7.1 years ago ribioinfo ▴ 100
3
votes
5
replies
5.6k
views
SingleCellExperiment function in SingleCellExperiment package returns error (when reproducing example)
R bioconductor singlecellexperiment single cell
updated 6.6 years ago by • 0 • written 6.6 years ago by ▴ 10
2
votes
2
replies
1.0k
views
normalization for infected homogenious cell populations
normalization single cell scran
updated 5.2 years ago by ★ 28k • written 5.2 years ago by • 0
2
votes
0
replies
1.7k
views
News: Ascona Workshop 2017 - Statistical Challenges in Single-Cell Biology: Deadline on 20 January
conference single cell statistical modeling News
7.6 years ago • updated 7.4 years ago Wolfgang Huber ★ 13k
2
votes
4
replies
1.3k
views
Impose minimum restrictions on correlations in SingleR
single cell R SingleR
updated 4.3 years ago by ★ 28k • written 4.3 years ago by ▴ 120
2
votes
3
replies
3.2k
views
scran: question on minimum cluster size for computeSumFactors()
scran single cell normalization clustering
8.1 years ago aatsmith • 0
2
votes
2
replies
1.2k
views
another contrast question - edgeR + scRNA-seq + timecourse
edger single cell design and contrast matrix timecourse
updated 6.0 years ago by 50k • written 6.0 years ago by • 0
2
votes
5
replies
1.9k
views
sc3_prepare function error
sc3 scater single-cell single cell
updated 7.3 years ago by ▴ 150 • written 7.3 years ago by ★ 1.5k
2
votes
3
replies
1.3k
views
splatter simulating paths
splatter single cell
6.1 years ago nikolaspapadop ▴ 10
1
vote
1
reply
1.3k
views
Does SingleR return output for every cell it's given or only those that it can provide an annotation for?
SingleR Single Cell
updated 3.6 years ago by ★ 28k • written 3.6 years ago by • 0
1
vote
3
replies
2.3k
views
Does it make sense to consider RUVr normalization for single cell RNA analysis?
RUV ruvseq ruvr ruvnormalize single cell
6.8 years ago Nik Tuzov ▴ 80
1
vote
0
replies
1.4k
views
Job: Scientific Programmer - Cambridge, UK
single cell job software engineer c++ Job
6.6 years ago Aaron Lun ★ 28k
1
vote
4
replies
2.1k
views
Trajectory analysis for single cell sequencing
single cell trajectory analysis tscan monocle
updated 3.8 years ago by ★ 28k • written 3.8 years ago by ▴ 40
1
vote
0
replies
946
views
Job: Postdoc/Bioinformatician Research Fellow @ Harvard Medical School/MGH/ Boston's Children Hospital
CRISPR functional genomics single cell gene regulatory networks Job
3.8 years ago bauer ▴ 10
1
vote
2
replies
1.3k
views
using gene list for singleR
single cell singelR
updated 3.7 years ago by ★ 28k • written 3.7 years ago by ▴ 40
1
vote
3
replies
1.3k
views
Raw counts for reference dataset in single cell rnaseq
single cell celldex singleR
updated 3.7 years ago by ★ 28k • written 3.7 years ago by ▴ 80
0
votes
1
reply
1.1k
views
How can I compute AIC for MAST hurdle model fitted in the whole dataset?
MAST AIC calcuation Parameters single cell hurdle model
updated 8 weeks ago by ▴ 20 • written 5.3 years ago by • 0
0
votes
7
replies
7.6k
views
Regarding scRNA seq vs Bulk RNA seq
single cell rnaseq
updated 6.7 years ago by ▴ 10 • written 6.7 years ago by • 0
0
votes
3
replies
1.3k
views
Batch effects and model matrices
deseq2 single cell monocle
updated 6.9 years ago by 42k • written 6.9 years ago by • 0
0
votes
2
replies
875
views
Celaref error relating to MAST DE ananlysis
software error celaref single cell RNA-seq
updated 5.0 years ago by ▴ 40 • written 5.0 years ago by • 0
0
votes
0
replies
857
views
Job: Post-doctoral Fellow - Aparicio Lab @ BC Cancer Research Center, Vancouver BC Canada
single cell genome cancer Job
5.0 years ago jmaanaki • 0
0
votes
0
replies
821
views
Job: Post-doctoral Fellowship at BC Cancer Research Center, Vancouver BC
single cell RNA Data analysis Bioinformatics Job
4.9 years ago jmaanaki • 0
0
votes
0
replies
929
views
Job: Post-doc fellow - Aparicio Lab
single cell bioinformatics genome sequencing Job
4.7 years ago jmaanaki • 0
0
votes
0
replies
1.2k
views
SCDE for differential expression
scde differential expression single cell
7.8 years ago mnaymik ▴ 10
0
votes
0
replies
1.3k
views
Job: Bioinformatics position at the Department of Genetics, Stanford, California
next-generation sequencing rna-seq chip-seq single cell Job
8.0 years ago Katja Hebestreit ▴ 130
0
votes
0
replies
1.7k
views
Job: PhD Studentship: Genome-scale analysis of host-pathogen interactions
rnaseq single cell bioinformatics R epigenomics Job
8.6 years ago Garry Myers • 0
0
votes
4
replies
1.2k
views
Issues with normalizing viral-infected single cell data
scan scater single cell normalization
updated 4.1 years ago by ★ 28k • written 4.1 years ago by ★ 2.0k
0
votes
0
replies
1.4k
views
News: C1omics: single-cell omics methods and applications, Manchester 24-25 Nov 2015
single cell conference workshop News
8.6 years ago Wolfgang Huber ★ 13k
0
votes
0
replies
1.7k
views
Job: Single Cell Cancer Genomics
cancer single cell genomics Job
9.4 years ago crispin.miller • 0
0
votes
4
replies
1.3k
views
marker genes in single cell experiment
single cell singlecellexperiment gene markers
updated 3.7 years ago by ★ 28k • written 3.7 years ago by ▴ 40
0
votes
0
replies
753
views
Single cell WGS and ATAC-seq tools
single cell singlecellexperiment atac seq WGS
3.7 years ago lirongrossmann ▴ 80
0
votes
0
replies
1.1k
views
News: SCANGEN: Single-cell cancer genomics workshop at ISMB 2018
single cell cancer genomics statistics News
6.1 years ago kieranrcampbell ▴ 50
0
votes
2
replies
1.3k
views
BASiCS spike info for single cell RNA sequences or Bayesian statistics help
BASiCS bayesian single cell scrnaseq
updated 6.3 years ago by ▴ 10 • written 6.3 years ago by ▴ 10
0
votes
0
replies
1.1k
views
Job: Postdoctoral position, T follicular helper cell transcriptomics
single cell rnaseq T cells Tfh cells immunology Job
5.9 years ago michela.locci • 0
0
votes
0
replies
1.4k
views
Job: Post-doc / Research Engineer Position: single cell RNAseq R/Perl applications development, IPMC, Sophia Antipolis, France
single cell Job
6.4 years ago Agnes Paquet ▴ 10
47 results • Page 1 of 1
Recent ...
Replies
Answer: Numerical differences in DESeq2 output depending on different design matrix cons by Michael Love 42k
Yes there can be small numerical differences in the estimated quantities because of how the algorithms decide to stop iterating.
Answer: DESeq2- Mysterious interaction suppresses DEGs by Michael Love 42k
Removing samples from one group nevertheless changes the variance estimation (dispersion) for all comparisons. So you could have pushed one…
Comment: Ensembl plants at annotationHub? by Daniele • 0
Hi, I'm using Ensembl Plants release 59 with the IWGSC RefSeq v1.1 gene annotation. Thanks a lot for replying so quickly!
Comment: Numerical differences in DESeq2 output depending on different design matrix cons by swbarnes2 ★ 1.4k
If you restrict yourself to, say, genes with a baseMean > 50, are the numerical differences between the two designs significant?
Comment: DESeq2- Mysterious interaction suppresses DEGs by shaunpeterson • 0
The images I tried to attach didn't attach. Here is the PCA (MDS) of all the samples, with the Cortex outlier highlighed. ![enter image de…
Votes
Answer: Error while installing BiocManager::install("org.Vdahliae.eg.db")
Comment: How can I create a eigencorplot of pca created from deseq2 object with PCAtools
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Rsubread counting peaks ATACseq
Awards • All
Popular Question to Vincent J. Carey, Jr. 6.7k
Popular Question to Koen Van den Berge ▴ 180
Popular Question to shepherl 3.9k
Popular Question to Ken C • 0
Popular Question to Johannes Rainer ★ 2.0k
Locations • All
The city by the bay, 35 minutes ago
UPF, Barcelona, Spain, 38 minutes ago
United States, 48 minutes ago
Sweden, 58 minutes ago
EMBL Heidelberg, 1 hour ago
Traffic: 388 users visited in the last hour
Content Search
Users
Tags
Badges
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6